2005 Conference Abstracts

*Fisher, D. J., M. Miyakawa, S. Sayeed, R. Poon, J. I. Rood, F. Uzal & B. A. McClane. Dissecting the role of Clostridium perfringens type B and C toxins in lethality using a mouse intravenous injection model", Microbial Pathogenesis and host response, Cold Spring Harbor, Sept 14-18 (2005).

*Sheedy, S.A., Rood, J. & Moore, R.J. Live vectored delivery of therapeutic and prophylactic proteins to the gut of chickens, Avian gut function health and disease - 28th Poultry Science Symposium, World's Poultry Science Association UK Branch, Bristol, UK, September (2005).

*Bannam, T.L., W.L. Teng, D. Bulach, D. Lyras & J.I. Rood. Functional biology of the conjugative R-plasmid from Clostridium perfringens, 3rd Conference on Functional Genomics of Gram-Positive Microorganisms, San Diego, USA, 12-16 June, (2005) T39.

*Cullen PA, Cordwell SJ, Adler B & Cameron CE. The leptospiral surfaceome and glycoproteome, Proc 4th Mtg Int Leptospirosis Soc, 112, Chiang Mai, Thailand (2005).

*Adler B, Bulach DM, Cullen PA, Davies JK, Rood JI, Coppel RL, Seemann T, Zuerner RL, Alt D, McGrath A, Wilson P & Kuczek E. What can we learn from comparative genomics of Leptospira? Proc 4th Mtg Int Leptospirosis Soc, 130, Chiang Mai, Thailand (2005).

*Boyce JD, Harper M, Steen J, Cullen PA, Wilkie I, Paustian M, Kapur V & Adler B. Genomic approaches to identifying novel Pasteurella multocida virulence genes, Pasteurellaceae 2005, 16, Hawaii, USA (2005).

*St Michael F, Harper M, Adler B & Cox AD. Lipopolysaccharide core oligosaccharides from the Heddleston serotypes of P. multocida: structural and genetic analyses, Pasteurellaceae 2005, 64, Hawaii, USA (2005).

*Steen JA, Harper M, Wilkie I, Adler B & Boyce JD. Signature-tagged mutagenesis of P. multocida in the natural chicken host using a trans-mucosal infection model, Pasteurellaceae 2005, 71, Hawaii, USA (2005).

*Harper M, Boyce JD, St Michael F, Wilkie I, Cox AD & Adler B. Structural and functional analysis of P. multocida LPS mutants. Pasteurellaceae 2005, 72, Hawaii, USA (2005).

*Ottesen JR, Olsen JE, Adler B & Boyce JD. Identification and characterisation of P. multocida stress genes, Pasteurellaceae 2005, 72, Hawaii, USA (2005).

*Witchell T, Bulach D, Coutts S, Cullen P & Adler B. Expression of the vsp genes and Vsp proteins of Brachyspira hyodysenteriae, Third Int. Conf. Colonic Spirochaetal Infections in Animals and Humans, 4, Parma, Italy (2005).

*Mijaljica D., Rosado, C., Devenish, R.J. & Prescott, M. Vacuolar turnover of mitochondria in cells lacking ATG products, Yeast, 22 (S1), S135 (10-4), Abstracts of the XXII International Conference on Yeast Genetics and Molecular Biology, Bratislava, Slovak Republic, August 7-12, (2005).

*T.M. Hansen, D.J. Smith & P. Nagley. Impaired release of death-signalling proteins from mitochondria in mouse cells null for cytochrome c, Proc. Aust. Soc. Biochem. Mol. Biol, 37, SYM-41-4, Adelaide (2005).

*Parker, D., R.M. Kennan & J.I. Rood. Regulation of type IV fimbriae in Dichelobacter nodosus, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p28.

*Sheedy, S. A., A.B. Ingham, J.I. Rood & R.J. Moore. Alpha toxin sequences of avian isolates of Clostridium perfringens, Australian Poultry Science Symposium 2005, Sydney, NSW.

*Han, X., R. M. Kennan, J.K. Davies & J.I. Rood. Differential effects of pilT and pilU mutations in Dichelobacter nodosus, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p27.

*Bannam, T.L., D. Bulach, D. Lyras & J.I. Rood. Complete nucleotide sequence and identification of the Rep protein of the conjugative R-plasmid pCW3 from C. perfringens, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p4.

*Teng, W.L., T.L. Bannam, D. Lyras & J.I. Rood. Molecular analysis of the conjugation region of the R-plasmid pCW3 from Clostridium perfringens, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p36.

*Rood, J.I. , R. M. Kennan, X. Han, D. Parker, D. Wong, J.K. Davies, O.P. Dhunyel, R.J. Whittington & J. R. Egerton. Fimbrial biogenesis, protease secretion and virulence in the ovine footrot pathogen Dichelobacter nodosus, Annual Scientific meeting of the Australian Society for Microbiology, 25-29 September, (2005).

*Sayeed, S., R. Poon, M. Miyakawa, D. J. Fisher, V. Adams, J.I. Rood, F. Uzal & B. A. McClane. Epsilon toxin levels in Clostridium perfringens type D cultures correlates with mouse lethality, Annual Scientific meeting of the Australian Society for Microbiology, 25-29 September, (2005).

*Parsons, J., T.L. Bannam, R. Devenish & J.I. Rood. The tcpA gene is required for the conjugative transfer of pCW3 in C. perfringens, Annual Scientific meeting of the Australian Society for Microbiology, 25-29 September, (2005).

*Boyce J, Cullen P, Nguyen V, Wilkie I & Adler B. Analysis of the P. multocida outer membrane sub-proteome and its response to the in vivo environment of the natural host, Eighth Aust. Bact. Pathog. Mtg. 23, Couran Cove, Australia. (2005).

*Harper M, Boyce J, Cox A & Adler B. Structural and functional analysis of P. multocida LPS mutants, Eighth Aust. Bact. Pathog. Mtg. 36, Couran Cove, Australia. (2005).

*Lo M, Boyce J & Adler B. Characterisation of a putative transcriptional regulator from P. multocida, Eighth Aust. Bact. Pathog. Mtg. p20, Couran Cove, Australia. (2005).

*Han, X., R.M. Kennan, J.K. Davies & J.I. Rood. Differential effects of pilT and pilU mutations in Dichelobacter nodosus, 8th Australian Bacterial Pathogenesis Conference, Couran Cove, Queensland, May (2005).

Lyras, D., S.A Ballard, W.L. Teng, V. Adams, P. Howarth, P.K. Crellin, T.L. Bannam, J.G. Songer & J.I. Rood. Identification of a plasmid encoded lincomycin resistance transposon in Clostridium perfringens, XI International Congress of Bacteriology and Applied Microbiology, San Francisco, July 23-28, (2005), 18-B-1196.

Henry RM, Bulach DM & Adler B. Identification and characterisation of leptospiral extracellular proteases, Proc 4th Mtg Int Leptospirosis Soc, 114, Chiang Mai, Thailand (2005).

Carrillo-Casas EM, Bulach DM, Adler B & de la Pena Moctezuma A. Regulation of transcription by the orfJ14-orfJ15 intergenic region of the Hardjoprajitno rfb locus, Proc 4th Mtg Int Leptospirosis Soc, 138, Chiang Mai, Thailand (2005).

Bulach DM, Cullen PA, Davies JK, Rood JI, Coppel RL, Seemann T, Zuerner RL, Alt D, McGrath A, Wilson P, Kuczek E & Adler B. The genome of Leptospira biflexa, Proc 4th Mtg Int Leptospirosis Soc, 136, Chiang Mai, Thailand (2005).

Lo M, Bulach DM, Haake DA, Matsunaga J, Paustian ML, Zuerner RL & Adler B. A analysis of the effects of temperature shifts on leptospiral gene expression as assessed by whole genome microarray, Proc 4th Mtg Int Leptospirosis Soc, 143, Chiang Mai, Thailand (2005).

Rodriguez-Reyes EA, Cullen PA, Bulach DM, Haake DA, Adler B & de la Pena Moctezuma A. Expression of GspD, a type II secretion system protein from Leptospira borgpetersenii serovar Hardjo, Proc 4th Mtg Int Leptospirosis Soc, 294, Chiang Mai, Thailand (2005).

Faux N, Bottomley S, Lesk A, Irving J, Morrison J, Garcia de la Banda G & Whisstock J. Functional insights from the distribution and role of homopeptide repeat containing proteins, VIth European Symposium of The Protein Society (Barcelona, Spain). April/May (2005).

Buckle AM, Zhang Q, Cabrita LD, Fulton KF, Irving JA, Butcher RE, Smith I, Reeve S, Ruzyla K, Pike R, Lesk AM, Rossjohn J, Bottomley SP & Whisstock JC. Getting hot and sticky: Structural dissection of serpins from thermophiles. Characterisation of a serpin from the hyperthermophilic archaeon, pyrobaculum aerophilum. The crystal structure of the human tumour suppressor maspin reveals a novel conformational switch in the G-helix, 4th International Symposium on Serpin Structure, Function and Biology, Cairns, Australia, June (2005).

Whisstock JC, Zhang Q, Law RHP, Ruzyla K, Irving JA, Cabrita L, Fulton KF, Lesk AM, Buckle AM, Rossjohn J & Bottomley SP. Obeying Anfinsen: a serpin that folds to the most stable state, XX Congress of the International Union of Crystallography, Florence, Italy August (2005).

Whisstock JC & Zhang QW. Evolution of metastability of serpins, 4th General Meeting of the International Proteolysis Society (Quebec, Canada). October (2005).

Bulach, D., T. Seemann, P. Wilson, A. McGrath, J. Davis, E. Kuczek, R. Coppel ,J. Davies, J. Rood, D. Alt, R. Zuerner & B. Adler. The Leptospira biflexa genome, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p32.

Adams, V., I.S. Lucet, F.E. Tynan, M. Chiarezza, P. Howarth, J.Kim, J. Rossjohn, D. Lyras & J.I. Rood. Identification of a DNA binding domain of the large serine recombinase TnpX, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p2.

Lyras, D., S.A Ballard, W.L. Teng, V. Adams, P. Howarth, P.K. Crellin, T.L. Bannam, J.G. Songer & J.I. Rood. Identification of a lincomycin resistance transposon Tn4454 in Clostridium perfringens, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11 (2005), p22.

Henry R, Bulach D & Adler B. Zymographic identification of extracellular leptospiral proteases, Eighth Aust. Bact. Pathog. Mtg. p14. Couran Cove, Australia (2005).

Lazar Adler N, Govan B, Harper M, Adler B & Boyce J. Elucidation of the molecular basis of pathogenesis of melioidosis by signature-tagged mutagenesis, Eighth Aust. Bact. Pathog. Mtg. p19. Couran Cove, Australia (2005).

P.M. Beart, M.L.R. Lim, B. Chen, D.J. Smith, P. Nagley & S. Diwakarla. Diverse insults release mitochondrial apoptogenic proteins despite differential actions on caspases and cellular calcium, Proc. Aust. Neurosci. Soc, 16, POS-TUES-279, Perth (2005).

P. Nagley, M.L.R. Lim & P.M. Beart. Differential release of death-signalling proteins from mitochondria during apoptosis in embryonic cortical neurones, Proc. Aust. Neurosci. Soc. 16, POS-TUES-356, Perth (2005).

L.D. Mercer, M.L.R. Lim, M.K. Horne, P. Nagley and P.M. Beart. Mitochondria, apoptosis and mesencephalic dopamine neurones: first evidence for AIF-mediated, capase-independent injury. Proc. Aust. Neurosci. Soc. 16, POS-TUES-357, Perth (2005).

L. Cheng, R. Steel, R.L. Anderson, D.J. Smith & P. Nagley. Heat shock proteins and neuroblastoma cell death: comparison of undifferentiated and neurone-like SH-SY5Y cells, Proc. Aust. Soc. Biochem. Mol. Biol. 37, POS-TUE-23, Adelaide (2005).

Howden, B., P. Johnson, P. Ward & J. Davies. Microarray analysis of hetero-vancomycin intermediate Staphylococcus aureus, 8th Australian Bacterial Pathogenesis Conference, Couran Cove, Queensland, May (2005).

Bulach, D., T. Seeman, P. Wilson, A. Mc Grath, J. Davies, E. Kuczek, R. Coppel, J. Davies, J. Rood, D. Alt, R. Zuerner & B. Adler. The Leptospira biflexa genome, 8th Australian Bacterial Pathogenesis Conference, Couran Cove, Queensland, May (2005).

Ryan, C.S., J.C. Whisstock, J. Cheung & J.K. Davies. Transcriptional regulation of the pilE gene of Neisseria gonorrhoeae, 8th Australian Bacterial Pathogenesis Conference, Couran Cove, Queensland, May (2005).

Turner, S.A., C. Kahler & J.K. Davies. Isolation of an ihf mutant and use of microarrays to assess the role of IHF in Neisseria meningitidis gene regulation, 8th Australian Bacterial Pathogenesis Conference, Couran Cove, Queensland. May (2005).

Davies, J. Neisseria meningitidis: an ever-changing pathogen, Annual Conference of the Australian Society for Microbiology, Canberra, ACT. September, (2005).

Young. L.F., M.M. Awad, R.L. Boyd, J.J. Emmins, M.J. Hickey & J.I. Rood. Response of the microvasculature to Clostridium perfringens toxin,  8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p20.

Cheung, J.K., M.M. Awad, H. Newton & J.I. Rood. Analysis of the VirS/VirR phosphorelay cascade from Clostridium perfringens, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p7.

Chiarezza, M., S. J.A. Pidot, M.M. Awad, D. Lyras & J.I. Rod. The sialidases of Clostridium perfringens and Clostridium septicum, 8th Australian Bacterial Pathogenesis Meeting, Couran Cove, Qld, May 8-11, (2005), p8.

O’Connor, J.R.., D. Lyras, J.K. Cheung, K.A Farrow & J.I. Rood. Functional analysis of the C. difficile VirR homologues RR17 and R55, 3rd Conference on Functional Genomics of Gram-Positive Microorganisms, San Diego, USA, 12-16 June, (2005), p90.